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By Bancroft W. D.

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In variable volume fed batch, the volume changes with the fermentation time owing to the substrate feed. The oldest and first industrial application of a fed-batch operation was the production of yeast cells in which glucose was added intermittently during fermentation to maintain a low sugar concentration to suppress the formation of alcohol [5]. High malt concentration favors cell growth, which in turn causes anaerobic conditions that favor ethanol production and lower the yield of yeast cells.

Lee, Metabolic engineering of Escherichia coli for the production of putrescine: a four carbon diamine, Biotechnology and Bioengineering 104 (2009) 651e662. [177] F. C. L. Cooney, Influence of environmental factors in the production of R(S)-1,2-propanediol by Clostridium thermosaccharolyticum, Biotechnology Letters 9 (1987) 449e454. [178] R. R. Nielsen, Styrene biosynthesis from glucose by engineered Escherichia coli, Metabolic Engineering 13 (2011) 544e554. [179] S. Okino, R. Noburyu, M. Suda, T.

A simple strategy of repeated batch cultivation for the enhanced production of 1,3-propanediol using Clostridium diolis was reported by Kaur et al. [4]. In a repeated operation, 20% (v/v) of culture broth was removed from the reactor and supplemented with an equal volume of fresh nutrient medium when the residual glycerol was below 15 g/L. 04 g/L/h were achieved. 5-fold, respectively, compared with batch fermentation. 2 Fed-Batch Fed-batch fermentation is a process in which one or more nutrients are fed to the bioreactor during cultivation and the products remain in the bioreactor until the end of the run.

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