By Bhanu P. Jena
This is often the 1st e-book to hide the historical past, constitution, and alertness of atomic strength microscopy in mobile biology. provided within the transparent, well-illustrated sort of the Methods in telephone Biology sequence, it introduces the AFM to its readers and allows them to faucet the facility and scope of this know-how to additional their very own study. a realistic laboratory advisor to be used of the atomic strength and photonic strength microscopes, it presents up-to-date expertise and techniques in strength spectroscopy. it's also a finished and easy-to-follow functional laboratory consultant for using the AFM and PFM in organic learn.
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Additional info for Atomic Force Microscopy in Cell Biology, Volume 68
Exposure of acinar cells to cytochalasin B, a fungal toxin that inhibits actin polymerization, results in a 50–60% loss of stimulation of amylase secretion. A signiﬁcant decrease in the depression diameter was observed following the treatment of acinar cells with cytochalasin B. Since the AFM uses force to image objects, it was unclear whether pits and depressions were actual surface structures at the plasma membrane or represented an arrangement of underlying cytoskeletal elements at the apical end of pancreatic acinar cells.
20 J. K. Heinrich Horber ¨ Fig. 12 Schematic drawing of the setup built for whole cell tissue patch-clamp/AFM applications. A piezotube scanner P1 moves the cantilever mounted on a titanium arm in xy and z directions. An xz translation stage allows the lateral and vertical coarse positioning of the cantilever. Additionally, an xy translation device moves the optical microscope. Thus, in combination with the vertical objective translation stage of the optical microscope, the laser beam is adjustable in all three orthogonal directions relative to the cantilever.
Czerny, C. , and Mayr, A. (1992). Scan. Microsc. 6, 919. H¨orber, J. K. , Lang, C. , H¨ansch, T. , Heckl, W. , and M¨ohwald, H. (1988). Chem. Phys. Lett. 145, 151. H¨orber, J. K. , and Sakmann, B. (1995). Biophys. J. 68, 1687. H¨orber, J. K. , Schuler, F. , and Ruppersberg, J. P. (1991). In “Scanned Probe Microscopies: STM and Beyond” (H. ), p. 241. AIP Conference Proceedings. H¨orber, J. K. , Schuler, F. , Schr¨oter, K. , and Ruppersberg, J. P. (1991). J. Vac. Sci. Technol. B9, 1214. , and Hudspeth, A.