Download Biotechnology in Agriculture: Proceedings of the First by Li Zhensheng (auth.), Chongbiao You, Zhangliang Chen, Yong PDF

By Li Zhensheng (auth.), Chongbiao You, Zhangliang Chen, Yong Ding (eds.)

The First Asia --- Pacific convention on Agricultural Biotechnology used to be held in Beijing, China on 20-24, August, 1992. Over part the inhabitants on this planet is within the Asian and Pacific zone. With an expanding inhabitants and lowering farming lands, it is very important enhance agricultural biotechnology for development of the productiveness, profitability and balance of the farming approach. The Conference's major ambitions have been to compile scientists operating in numerous fields of agricultural biotechnology to stimulate dialogue in this vital strategy and to have an appraisal of the newest reports touching on genetic manipulation of crops, plant telephone and tissue tradition, plant gene legislation, plant-microbe interplay, animal biotechnology and so on. The convention used to be attended by means of 391 scientists from assorted international locations and areas. This quantity offers the contributions of the lectures and a specific variety of posters, that are an updated account of the country of information on agricultural biotechnology. The e-book presents a necessary reference resource not just for experts in agricultural biotechnology, but additionally for researchers engaged on comparable features of agronomy, biochemistry, genetics, molecular biology, microbiology and animal sciences. it truly is with nice excitement to recognize the contributions of the authors in assuring the advised ebook of this quantity. we might additionally expand our honest thank to Kluwer educational Publishers for the e-book of those proceedings.

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Additional info for Biotechnology in Agriculture: Proceedings of the First Asia-Pacific Conference on Agricultural Biotechnology, Beijing, China, 20–24 August 1992

Example text

The same probe was used to confirm hybrid nature of somatic fusion plants of D. catholica + B. juncea, B. spinescens +B. juncea, T. ballii +B. campestris (Kirti et al. unpublished) and somatic hybrids between C. sativa + B. carinta (Narasimhulu et al. unpublished). Molecular analysis of B. carinata synthesized by protoplast fusion of parental species was made by probing with a B. juncea derived genomic clone BJG 425 (Mohaptra 1991). The clone binds differentially to Hind III fragments of the two diploid parental species.

Ballii+B. juncea all other hybrid combinations showed mitochondria predominantly from the cultivated parent. Mixed mitochondrial DNA sequences of both parental species were observed in somatic hybrids B. Juncea(Kirti et al unpublished). juncea. Approximately 28% of all hybrid analysed, and more than 50% of the hybrids in these combinations, showed mitochondrial rearrangements (Table 4). Protoplast fusion thus provides a unique oIJportunity of recovering novel mitochondrial gene combinations which could not have been possible by conventional hybridisation.

Pollen mother cells had normal meiosis with 17 bivalents. Occasional cells had upto 3 quadrivalents or 4 univalents. Pollen fertility varied widely, ranging from 36 to 87%, while seed fertility centered around 19%. DNA analysis Nuclear genome: Molecular confirmation of hybrid nature of regenerants from somatic fusion products was done using restrication fragment length polymorphism. Southern hybridization patterns were estabished initially by restricting DNA samples of 20 parental species with various restriction enzymes and probing with a nick translated plasmid DNA TA 71 carrying full length wheat nuclear rDNA sequence (Gerlach and Bedbrook 1979).

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